BRAF p.Val600Glu

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The BRAF Mutation Analysis Kit is an in vitro diagnostic device intended for the detection of BRAF codon 600 GTG>GAG (V600E) mutation in human genomic DNA isolated from tumor tissues of patients diagnosed with melanoma and other cancers.

The BRAF p.Val600Glu kit is CE marked and can be used for human in vitro diagnostic tests.

BRAF gene codes for B-Raf protein, a member of the Raf kinase family of growth signal transduction protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Codon 600 in exon 15 of the gene is frequently mutated in various cancers. The mutations result in constitutively active form of the protein that may lead to tumorigenesis and cancer progression.

Patients with advanced melanoma harbored by BRAF codon 600 mutations are likely to experience clinical benefit from kinase inhibitor drugs that target the mutant variants of the protein. This kit is usefull to guide therapeutic decisions for patients with advanced melanoma.

Kit contains lyophilized sequence-specific primers and TaqMan probes for BRAF V600E mutation (generating FAM signal) and internal control amplifying part of globin gene (generating JOE signal) to be used in real time PCR.

Each assay is able to detect 1% mutation in a background of wild-type DNA in the total amount of 20 ng of DNA. (Davies, 2014; Hertzman, 2014; Joseph, 2010).




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Information for use

BRAF p.Val600Glu kit


25 rxn

upon request





    • Davies MA. Finding the Right Balance of BRAF Inhibition in Melanoma. Cancer Discov 2014;4(5):510-512.
    • Hertzman JC, Egyhazi BS. BRAF inhibitors in cancer therapy. Pharmacol Ther 2014;142(2):176- 182.
    • Joseph EW, Pratilas CA, Poulikakos PI, Tadi M, Wang W, Taylor BS, Halilovic E, Persaud Y, Xing F, Viale A, Tsai J, Chapman PB, Bollag G, Solit DB, Rosen N. The RAF inhibitor PLX4032 inhibits ERK signaling and tumor cell proliferation in a V600E BRAF-selective manner. Proc Natl Acad Sci U S A 2010;107(33):14903-14908.