The bioanalytical method of Fluorescence In Situ Hybridization (FISH) is widely used to determine genetic aberrations in normal and tumor tissues or cells. It can be applied for both the mitotic chromosomes and interphase nuclei. The technique is considered as one of the most precise and still routinely applicable methodologies used for analysis of gene alterations. Currently, the FISH is used for research and diagnostics of hereditary genetic diseases and, more frequently, in analysis of (un)balanced chromosomal alterations in human cancers. IntellMed product line offers a wide range of directly labeled fluorescent DNA probes for the determination of gene status or for chromosome enumeration in human tissues. The probes are offered in the two colors (orange and green), or as a dual variant with combination of these two fluorophores.
List of offered probes
The detection of circulating tumor cells (CTCs) is important in determining early stages of metastases, the so called micro-metastases, for tumour diseases which cannot be monitored with usual visualization methods (tomography, magnetic resonance, ultrasonic examination). Tumour cells isolated from blood, bone-marrow or the lymphatic system of the patient are detected, and these cells are considered precursors of micro-metastases. The principle of the most sensitive method for the determination of CRCs is the real-time reverse transcription polymerase chain reaction (real-time RT-PCR), that makes it possible to detect down to one tumour cells among ten millions of non-tumour cells.
List of markers for the detection of CTCs
Product Use Limitation:
IntellMed products are "For Research Use Only,not for Diagnostic or Therapeutic Use" unless otherwise stated.