LSI 17p11.2 (Green, Orange)

Probe location

17p11.2

Probe description

The LSI 17p11.2 FISH kit is intended for the enumeration of chromosome 17 in human tissues using fluorescence in situ hybridization (FISH).
The LSI 17p11.2 FISH kit contains a directly labeled fluorescent DNA probe in hybridization buffer. The fluorochrome Orange or Green labeled probe is targeted to the chromosome 17 subcentromeric region.
The presence of false positive CEP17 signals, caused by non-specific hybridization of centromeric probe or complex karyotype, has been demonstrated. The 17p11.2 probe is used for the verification of the chromosome 17 copy number which is necessary for exact assessment of the Her-2/neu gene status (cross-hybridization versus polysomy).

FISH result

Figure1. A normal cell hybridized with the LSI 17p11.2 (Orange). The normal interphase cell in this image shows two orange signals (human lyphocytes).

Information

Name	Catalogue number	Packing size	Prize	Information for use
LSI 17p11.2 (Green, Orange)	IM_023	200μl	upon request	pdf

Reference

Tse CH, Hwang HC, Goldstein LC, Kandalaft PL, Wiley JC, Kussick SJ, Gown AM. Determining true HER2 gene status in breast cancers with polysomy by using alternative chromosome 17 reference genes: implications for anti-HER2 targeted therapy. J Clin Oncol. 2011 Nov 1;29(31):4168-74.