LSI Cyclin D1 (Orange)/CEP11 (Green)

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Probe location

Cyclin D1

Probe description

The LSI Cyclin D1 FISH kit is intended for the determination of Cyclin D1 gene amplification in human tissues using fluorescence in situ hybridization (FISH).
The Cyclin D1 FISH kit contains two directly labeled fluorescent DNA probes in hybridization buffer. The fluorochrome Orange labeled Cyclin D1 probe covers the chromosome 11q13 region. The fluorochrome Green labeled chromosome enumeration probe CEP11 covers the chromosome 11p11.1-q11.1 region.
The protein encoded by Cyclin D1 (CCND1) gene belongs to the highly conserved cyclin family. Cyclins and cyclin-dependent kinases (CDK) represent  the main regulatory systems of the cell cycle. The synthesis of Cyclin D1 is initiated during G1 and drives through the G1/S phase transition. Gene amplification or an increased production of the Cyclin D1 protein can be found in the different types of cancer, e. g. primary breast carcinoma, ovarian carcinoma, hepatocellular carcinoma, and small-cell lung carcinoma.

FISH results

A normal cell in FFPE tissue hybridized with LSI Cyclin D1 (Orange)/CEP11 (Green) shows 2 orange signals of Cyclin D1 gene and 2 green signals of chromosome 11. An abnormal cell in FFPE tissue hybridized with LSI Cyclin D1 (Orange)/CEP11 (Green) shows a higher copy number of Cyclin D1 gene and 2 green signals of chromosome 11.

Figure. 1: The assessment of Cyclin D1 gene copy number and copy number of chromosome 11 in the FFPE tissue.         
red LSI Cyclin D1 
green CEP11
a) 2 copies of Cyclin D1 gene and 2 copies of chromosome 11 in a normal cell (HL60S cell line).
b) 2 copies of chromosome 11 and higher copy number of Cyclin D1 gene (amplification) in an abnormal cell (SKMEL2 cell line).



Catalogue number

Packing size


Information for use

LSI Cyclin D1 (Orange)/CEP11 (Green)



upon request



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