LSI MDM2 (Orange)

« back

Probe location


Probe description

The LSI MDM2 FISH kit is intended for the determination of MDM2 gene status in human tissues using fluorescence in situ hybridization (FISH).
The LSI MDM2 FISH kit contains a directly labeled fluorescent DNA probe in hybridization buffer. The fluorochrome Orange labeled probe covers the chromosome 12q13-14 region.
The MDM2 (Murine Double Minute 2) gene encodes a protein composed of 491 amino acids which acts as an E3 ubiquitin ligase, forms a tight complex with the p53 protein and negatively regulates apoptosis. Amplification of MDM2, and thus inactivation of p53 pathway, is found in about 5-10 % of human tumors, including gliomas. The use of the MDM2 probe with other relevant markers (e.g. 1p36.3, 19q13, and 10p11.1) is important for the complex diagnostics of malignant gliomas.

FISH results

A normal cell hybridized with the LSI MDM2 (Orange) (1a). The cell in this image shows 2 orange signals. An abnormal cell (1b) shows more than 20 orange signals which indicate the MDM2 gene amplification.

A normal cell hybridized with the LSI MDM2 (Orange) shows 2 orange signals of the MDM2 gene. An abnormal cell hybridized with the LSI MDM2 (Orange) shows more than 20 orange signals which indicate the amplification of MDM2 gene.
1a 1b

Figure 1. The assessment of MDM2 status in FFPE tissue (glioma patient tissue).



Catalogue number

Packing size


Information for use

LSI MDM2 (Orange)



upon request



  • Eischen CM, Lozano G. p53 and MDM2: antagonists or partners in crime? Cancer Cell. 2009 Mar 3;15(3):171-83. [fulltext]
  • Chène P. Inhibiting the p53-MDM2 interaction: an important target for cancer therapy. Nat Rev Cancer. 2003 Feb;3(2):102-9. [fulltext]
  • Picksley SM, Spicer JF, Barnes DM, Lane DP. The p53-MDM2 interaction in a cancer-prone family, and the identification of a novel therapeutic target. Acta Oncol. 1996;35(4):429-34. [fulltext]