LSI RARA (Orange)

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Probe location


Probe description

The LSI RARA FISH kit is intended for the determination of RARA gene amplification and/or rearrangement in human tissues using fluorescence in situ hybridization (FISH).
The LSI RARA FISH kit contains a directly labeled fluorescent DNA probe in hybridization buffer. The fluorochrome Orange labeled RARA probe covers the chromosome 17q21 region.
RARA (Retinoic acid receptor alpha) gene codes for the retinoic acid receptor α which regulates transcription in a ligand-dependent manner. RARA gene is often co-amplified with Her-2/neu and TOP2A genes. It has already been evidenced that alterations in the RARA gene are associated with breast ductal carcinoma in situ (DCIS), positive estrogen receptors and high proliferation.

FISH results

A normal cell hybridized with the LSI RARA (Orange)/CEP17 (Green) shows 2 orange signals of the RARA gene and 2 green signals of chromosome 17. An abnormal cell hybridized with the LSI RARA (Orange) shows more than 20 orange signals which indicate the amplification of RARA gene.

Figure 1. The assessment of RARA gene copy number with the use of chromosome enumeration probe CEP17 (A) and without it (B) (breast cancer patient FFPE tissue).



Catalogue number

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Information for use

LSI RARA (Orange)



upon request



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  • Keith WN, Douglas F, Wishart GC, McCallum HM, George WD, Kaye SB, Brown R: Co-amplification of erbB2, topoisomerase II α  and retinoic acid receptor α  genes in breast cancer and allelic loss of topoisomerase I on chromosome 20. Eur J Cancer 1993; 29A(10): 1469-1475. [fulltext]
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